Biological Chemistry, 30 October 2019, DOI:https://doi.org/10.1515/hsz-2019-0311

Intracellular distribution of pseudorabies virus UL2 and detection of its nuclear import mechanism

Meili Li, Zuo Xu, Xingmei Zou, Yuanfang Wang, Yiwen Li, Xiaowen Ou, Yangxi Deng, Yingjie Guo, Weidong Gan, Daixiong Chen, Tao Peng, Jing Xiao and Mingsheng Cai*

Abstract

Pseudorabies virus (PRV) UL2 (pUL2) is a multifunctional protein, which is homologous with herpes simplex virus 1 early protein UL2 (hUL2) and crucial for the viral propagation. Yet, how pUL2 executes its roles in the viral life cycle remain inadequately understood. In order to uncover its effect on the procedure of PRV infection, investigation was performed to examine the subcellular distribution of pUL2 and establish its trafficking mechanism. In the present study, enhanced yellow fluorescent protein or Myc tag fused pUL2 was transiently overexpressed in transfected cells and exhibited an absolutely nuclear accumulation without the existence of other PRV proteins. Additionally, the nuclear trafficking of pUL2 was proved to rely on Ran-, transportin-1, importin β1, importin α1, α3 and α5. Accordingly, these data will benefit the knowledge of pUL2-mediated biological effects in PRV infection cycle.

文章链接：https://www.degruyter.com/view/j/bchm.2020.401.issue-2/hsz-2019-0311/hsz-2019-0311.xml