Vaccines, 17 April 2019, DOI:  https://doi.org/10.1016/j.vaccine.2019.03.031

Virus-like particle vaccines for poliovirus types 1, 2, and 3 with enhanced thermostability expressed in insect cells

Yuhua Xu, Shuzhi Ma, Yangbo Huang, Fengying Chen, Liyun Chen, Dong Ding, Yanping Zheng, Hua Li, Jing Xiao, Jin Feng, Tao Peng

1 Sino-French Hoffmann Institute of Immunology, State Key Laboratory of Respiratory Disease, College of Basic Medical Science, Guangzhou Medical University, Guangzhou, China 

2 Guangdong South China Vaccine Co. Ltd, Guangzhou, China

Abstract

Poliovirus (PV) is a pathogen that causes poliomyelitis, which may lead to paralysis and fatality. Inactivated PV vaccines (IPVs) and live-attenuated oral PV vaccines (OPVs) are currently used to defend against PV worldwide. Vaccines must be developed in a PV-free environment given the biosafety issues associated with OPV and IPV production and to eradicate PV globally. In this study, PV1, PV2, and PV3 virus-like particles with enhanced thermostability (PV-sVLPs) were produced in large quantities by using a baculovirus expression vector system (BEVS). Mice immunized with PV-sVLPs generated antibodies with strong PV-neutralizing response. In addition, splenocytes collected from immunized mice expressed high levels of IFN-c, IL-2, GM-CSF, IL-5, and IL-10 upon PV-sVLPs stimulation. These data suggest that PVsVLPs can serve as vaccines against PV infection.

文章链接：https://www.sciencedirect.com/science/article/pii/S0264410X19303536