个人概况

姓名：陈笛 性别：女

职称：研究员  导师资格：硕导

单位：广州医科大学广州霍夫曼免疫研究所

学历：博士研究生

毕业院校：University of Western Ontario

招生专业：基础医学、生化与分子生物学

电子邮件：chenseptember@outlook.com

联系方式：020-37105627

通信地址：广州医科大学番禺校区B1-207

研究方向：以果蝇为模型，研究与病毒感染以及肿瘤免疫识别及反应相关的天然免疫基因以及分子通路。

主持或参加科研项目及人才计划项目情况：

1. 国家自然科学基金委员会，青年科学基金项目，32200578，“mthl1作为潜在的新识别受体在宿主识别与应答中的功能和机制”，2023-01-01至2025-12-31，30万元，在研，主持。

2. 广东省科技计划项目（“海外名师”团队项目），109247859036，“以果蝇为模型研究天然免疫的抗肿瘤和病毒的分子机制”2021/09/01-2023/08/31、20万元、在研，主持。

3. 广东省科学技术厅，广东省科技计划项目（“海外名师"项目) ，以果蝇为模型探索天然免疫对原癌细胞的识别以及反应， 006012544080，2019-09 至 2021-08，2万元，结题，主持。

4. 广州市科学技术局，髓样来源抑制性细胞促进肝癌细胞干性的分子机制研究，广州市科技计划项目，201707010030，2017-05 至 2020-04，20万元，结题，主持。

5. 广东省自然科学基金委员会，沉默变异BRAF增加T细胞活性的机制研究，广东省自然科学基金博士启动项目，2016A030310278，2016-06 至 2019-06，10万元，结题，主持。

6. 广州市科学技术局，透皮沉默BRAF治疗黑色素瘤的免疫学机制研究，广州市科技计划项目，201505011546406，2016-05.01 至 2019-04.30，20万元，结题，主持。

7. 广州医科大学博士启动项目，20150021， “乳腺癌的RNA干扰免疫疗法：靶向沉默肿瘤相关巨噬细胞的克隆刺激因子受体以及肿瘤坏死因子的表达” 2015/01-2017/12、5万元、结题，主持。

8. 教育部、国家外国专家局，国家天然免疫机理研究学科创新引智基地，战略科技发展类("111"项目），D18010，2018-01 至 2023-01，450万元（约每年15万)，在研，参与。

发表文章列表：

1. Di Chen *, Xiao Lan , Xiaoming Huang , Jieqing Huang , Xiaojing Zhou , Jiyong Liu , Jules A Hoffmann*. Methuselah like 1, a Drosophila homologue of Mammalian adhesion GPCRs, is involved in antitumor reactions to injected oncogenic cells in flies. PNAS, 2023, (in press).

2. Di Chen*, Xiao Lan, Xiaoming Huang, Jieqing Huang, Xiaojing Zhou, Zhichao Miao, Yuting Ma, Akira Goto, Shanming Ji, Jules A. Hoffmann*. Single Cell Analysis of the Fate of Injected Oncogenic RasV12 Cells in Adult Wild Type Drosophila. Journal of Innate Immunity, 2023, 15:442–467.

3. Qingshuang Cai, Huimin Guo, Rong Fang, Yongzhi Hua, Yangyang Zhu, Xianrui Zheng, Jing Yan, Jiale Wang, Yixuan Hu, Chuchu Zhang, Chao Zhang, Renjie Duan, Fanrui Kong, Shikun Zhang, Di Chen*, Shanming Ji*. A Toll-dependent Bre1/Rad6-cact feedback loop in controlling host innate immune response. Cell Reports, 2022, 47.

4. Alexandre Cammarata-Mouchtouris, Adrian Acker, Akira Goto, Di Chen, Nicolas Matt *, Vincent Leclerc *. Dynamic Regulation of NF-κB Response in Innate Immunity:The Case of the IMD Pathway in Drosophila. Biomedicines, 2022, 10(2304).

5. Di Chen (#)*, Arghyashree Roychowdhury-Sinha, Pragya Prakash, Xiao Lan, Wenmin Fan, Akira Goto*, Jules A. Hoffmann*. A time course transcriptomic analysis of host and injected oncogenic cells reveals new aspects of Drosophila immune defences. PNAS, 2021, 118.

6. Chuqin Huang, Rui Xu, Samuel Liégeois, Di Chen, Zi Li, Dominique Ferrandon. Differential requirements for Mediator complex subunits in Drosophila melanogaster host defense against fungal and bacterial pathogens. Frontiers Immunology, 2021, 11.

7. Hua Cai, Andreas Holleufer, Bine Simonsen, Juliette Schneider, Aurélie Lemoine, Hans-Henrik Gad, Jingxian Huang, Jieqing Huang, Di Chen, Tao Peng, João T. Marques, Rune Hartmann, Nelson Martins, Jean-Luc Imler. "2'3'-cGAMP triggers a STING- and NF-kappaB-dependent broad antiviral response in Drosophila." Sci Signal，2020, 13(660).

8. Hua Cai, Wenxia Yao, Jingxian Huang, Jing Xiao, Wenli Chen, Longbo Hu, Runming Mai, Mengdi Liang, Di Chen, Nan Jiang, Li Zhou, Tao Peng. Apolipoprotein M, identified as a novel hepatitis C virus (HCV) particle associated protein, contributes to HCV assembly and interacts with E2 protein. Antiviral research, 2020, 177, 104756.

9. Li Zhou, Di Chen, Xu-Ming Huang, Fei Long, Hua Cai, Wen-Xia Yao, Zhong-Cheng Chen, Zhi-Jian Liao, Zhe-Zhi Deng, Sha Tan, Yi-Long Shan, Wei Cai, Yu-Ge Wang, Ri-Hong Yang, Nan Jiang, Tao Peng, Ming-Fan Hong, Zheng-Qi Lu. Wnt5a Promotes Cortical Neuron Survival by Inhibiting Cell-Cycle Activation. Frontiers in Cellular Neuroscience, 2017,281(11): 1-16.

10. Di Chen, James Koropatnick, Nan Jiang, Xiufen Zheng, Xusheng Zhang, Hongmei Wang, Keng Yuan, King Sun Siu, Aminah Shunnar, Colin Way, Wei-Ping Min. Targeted siRNA Silencing of Indoleamine 2, 3-Dioxygenase in Antigen-presenting Cells Using Mannose-conjugated Liposomes: A Novel Strategy for Treatment of Melanoma. Journal of immunotherapy, 2014, 37(2):123-34.

11. King Sun Siu, Di Chen, Xiufen Zheng, Xusheng Zhang, Nathan Johnston, Yanling Liu, Ken Yuan, James Koropatnick, Elizabeth R. Gillies and Wei-Ping Min(*). Single-walled carbon nanotubes non-covalently functionalized with lipid modified polyethylenimine for siRNA delivery in vitro and in vivo. Biomaterials, 2014, 35:3435-3442.

12. Siu KS(#), Zheng X, Liu Y, Zhang Y, Zhang X, Chen D, Yuan K, Gillies ER, Koropatnick J, Min WP(*). Single-walled carbon nanotubes noncovalently functionalized with lipid modified polyethylenimine for siRNA delivery in vitro and in vivo. Bioconjug Chem, 2014, 25(10):1744-51.

13. Maleki Vareki S(#), Rytelewski M, Figueredo R, Chen D, Ferguson PJ, Vincent M, Min W, Zheng X, Koropatnick J(*). Indoleamine 2,3-dioxygenase mediates immune-independent human tumor cell resistance to olaparib, gamma radiation, and cisplatin. Oncotarget, 2014, 5(9):2778-91.

14. Zheng X(#), Koropatnick J, Chen D, Velenosi T, Ling H, Zhang X, Jiang N, Navarro B, Ichim TE, Urquhart B, Min W(*). Silencing IDO in dendritic cells: a novel approach to enhance cancer immunotherapy in a murine breast cancer model. Int J Cancer, 2013, 132(4):967-77.

15. Jiang N(#), Zhang X, Zheng X, Chen D, Siu K, Wang H, Ichim TE, Quan D, McAlister V, Chen G, Min WP(*).A novel in vivo siRNA delivery system specifically targeting liver cells for protection of ConA-induced fulminant hepatitis. PLoS One, 2012, 7(9):e44138.

16. Li R(#), Zheng X, Popov I, Zhang X, Wang H, Suzuki M, Necochea-Campion RD, French PW, Chen D, Siu L, Koos D, Inman RD, Min WP(*). Gene silencing of IL-12 in dendritic cells inhibits autoimmune arthritis. J Transl Med, 2012, 10:19.

17. X. Zheng(#), J. Jiang, X. Zhang, D. Chen, B. Navarro, P. Luke, B. Garcia, A. M. Jevnikar, W. Min(*).  Reduction of liver ischemia reperfusion injury by silencing of TNF-α gene with shRNA. J Surg Res.2012, 176(2):614-20.

18. Zhang X(#), Beduhn M, Zheng X, Lian D, Chen D, Li R, Siu LK, Marleau A, French PW, Ichim TE, Min WP(*). Induction of alloimmune tolerance in heart transplantation through gene silencing of TLR adaptors. Am J Transplant. 2012, 12(10):2675-88.

19. Jiang N(#), Zhang X, Zheng X, Chen D, Zhang Y, Siu LK, Xin HB, Li R, Zhao H, Riordan N, Ichim TE, Quan D, Jevnikar AM, Chen G, Min W(*). Targeted gene silencing of TLR4 using liposomal nanoparticles for preventing liver ischemia reperfusion injury. Am J Transplant. 2011, 11(9):1835-44.

获奖经历:

1.国家留学奖学金2017-2019；

2.病理学年度研究日最佳基础研究海报陈述奖，2013/04；

3.加拿大健康研究院战略性培训癌症学术研究与实际应用转换项目奖学金，2009/09-2013/04；

4.加拿大健康研究院海报陈述比赛金奖（国家级），2012/06；

5.加拿大健康研究院战略性培训癌症学术研究与实际应用转换项目暨肿瘤系年度研究日最佳学术海报陈述奖，2010；

6.暨南大学研究生奖学金一等奖，2006-2007。